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KMID : 0352720080320030232
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Journal of Ginseng Research
2008 Volume.32 No. 3 p.232 ~ p.237
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Isolation and Characterization of Cinnamoyl-CoA Reductase Gene from Panax ginseng C. A. Meyer
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Parvin Shohana
Pulla Rama Krishna
Kim Yu-Jin
Yang Deok-Chun
Shim Ju-Sun
Jung Dea-Yeoung
Kim Se-Hwa
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Abstract
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Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) catalyses the reduction of cinnamic acid CoA esters into their corresponding aldehydes, the first step of the phenylpropanoid pathway specially dedicated to monolignol biosynthesis. A cDNA clones encoding CCR have been isolated from Panax ginseng C.A. Meyer and its expression was investigated in response to abiotic stresses. The cDNA, designated PgCCR which is 865 nucleotides long and has an open reading frame of 590 bp with a deduced amino acid sequence of 176 residues. The PgCCR encoded protein possesses substantial homology with CCRs isolated and cloned from other sources; the highest identity (51.8%) was observed with CCR from Tomato (Lycopersicon esculentum). Under various stress conditions, expression patterns of the PgCCR were highly induced in adventitious and hairy roots by several abiotic stresses. These results indicated that PgCCR plays protective role against diverse environmental stresses.
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KEYWORD
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Panax ginseng, abiotic stress, cinnamoyl-CoA reductase, salicylic acid, semi-quantitative RT-PCR
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